2blv

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 2blv, resolution 1.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

TRYPSIN BEFORE A HIGH DOSE X-RAY "BURN"

Overview

Specific radiation damage can be used to solve macromolecular structures, using the radiation-damage-induced phasing (RIP) method. The method has, been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets, that were collected on a third-generation synchrotron undulator beamline, with a highly attenuated beam. Each crystal was exposed to the, unattenuated X-ray beam between the collection of a 'before' and an, 'after' data set. The X-ray 'burn'-induced intensity differences ranged, from 5 to 15%, depending on the protein investigated. X-ray-susceptible, substructures were determined using the integrated direct and Patterson, methods in SHELXD. The best substructures were found by downscaling the, ... [(full description)]

About this Structure

2BLV is a [Single protein] structure of sequence from [Bos taurus] with CA, SO4, BEN and GOL as [ligands]. Active as [[1]], with EC number [3.4.21.4]. Full crystallographic information is available from [OCA].

Reference

Improving radiation-damage substructures for RIP., Nanao MH, Sheldrick GM, Ravelli RB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:16131756

Page seeded by OCA on Mon Oct 29 21:10:12 2007