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1u8v
From Proteopedia
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Crystal Structure of 4-Hydroxybutyryl-CoA Dehydratase from Clostridium aminobutyricum: Radical catalysis involving a [4Fe-4S] cluster and flavin
Overview
Dehydratases catalyze the breakage of a carbon-oxygen bond leading to, unsaturated products via the elimination of water. The 1.6-A resolution, crystal structure of 4-hydroxybutyryl-CoA dehydratase from the, gamma-aminobutyrate-fermenting Clostridium aminobutyricum represents a new, class of dehydratases with an unprecedented active site architecture. A, [4Fe-4S](2+) cluster, coordinated by three cysteine and one histidine, residues, is located 7 A from the Re-side of a flavin adenine dinucleotide, (FAD) moiety. The structure provides insight into the function of these, ubiquitous prosthetic groups in the chemically nonfacile, radical-mediated, dehydration of 4-hydroxybutyryl-CoA. The substrate can be bound between, the [4Fe-4S](2+) cluster and the FAD with both cofactors contributing to, its radical activation and catalytic conversion. Our results raise, interesting questions regarding the mechanism of acyl-CoA dehydrogenases, which are involved in fatty acid oxidation, and address the divergent, evolution of the ancestral common gene.
About this Structure
1U8V is a Single protein structure of sequence from Clostridium aminobutyricum with SF4 and FAD as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of 4-hydroxybutyryl-CoA dehydratase: radical catalysis involving a [4Fe-4S] cluster and flavin., Martins BM, Dobbek H, Cinkaya I, Buckel W, Messerschmidt A, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15645-9. Epub 2004 Oct 20. PMID:15496473
Page seeded by OCA on Wed Nov 21 03:55:39 2007
