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1ugy
From Proteopedia
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Crystal structure of jacalin- mellibiose (Gal-alpha(1-6)-Glc) complex
Overview
The structures of the complexes of tetrameric jacalin with Gal, Me-alpha-GalNAc, Me-alpha-T-antigen, GalNAcbeta1-3Gal-alpha-O-Me and, Galalpha1-6Glc (mellibiose) show that the sugar-binding site of jacalin, has three components: the primary site, secondary site A, and secondary, site B. In these structures and in the two structures reported earlier, Gal or GalNAc occupy the primary site with the anomeric carbon pointing, towards secondary site A. The alpha-substituents, when present, interact, primarily hydrophobically, with secondary site A which has variable, geometry. O-H..., centered pi and C-H...pi hydrogen bonds involving this, site also exist. On the other hand, beta-substitution leads to severe, steric clashes. Therefore, in complexes involving beta-linked, disaccharides, the reducing sugar binds at the primary site with the, non-reducing end located at secondary site B. The interactions at, secondary site B are primarily through water bridges. Thus, the nature of, the linkage determines the mode of the association of the sugar with, jacalin. The interactions observed in the crystal structures and modeling, based on them provide a satisfactory qualitative explanation of the, available thermodynamic data on jacalin-carbohydrate interactions. They, also lead to fresh insights into the nature of the binding of, glycoproteins by jacalin.
About this Structure
1UGY is a Protein complex structure of sequences from Artocarpus integrifolia. Full crystallographic information is available from OCA.
Reference
Structural basis of the carbohydrate specificities of jacalin: an X-ray and modeling study., Jeyaprakash AA, Katiyar S, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2003 Sep 5;332(1):217-28. PMID:12946359
Page seeded by OCA on Wed Nov 21 04:06:58 2007
