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Green Fluorescent Protein

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Background

Osamu Shimomura, Martin Chalfie and Roger Y. Tsien shared the 2008 Nobel Prize in Chemistry for their for the discovery and development of the green fluorescent protein, GFP.
GFP is small protein (21 kDa) and does not require cofactors to become fluorescent.

Structure

initial scene</scene>)' script='Green_Fluorescent_Protein/1ema_highlightchromophore/2'/>

1ema, resolution 1.90Å ()

Non-Standard Residues:

Structural annotation:
Resources:	CATH : 1Emaa00 InterPro : Ipr000786, Ipr011584, Ipr009017 Pfam : PF01353 SCOP : d1emaa_ UniProt : P42212

Evolutionary conservation:

Toggle Conservation Colors:	Rows = identical sequences:
Further Information:	Caveats, Explanation, Complete Results at ConSurfDB

Resources:

FirstGlance, OCA, RCSB, PDBsum

Coordinates:

save as pdb, mmCIF, xml

1ema, resolution 1.90Å ()

Coordinates:

save as pdb, mmCIF, xml

The crystal structure of GFP ^[1]^[2] is an eleven-stranded anti-parallel beta- barrel, threaded by an alpha-helix, running up along the axis of the cylinder.

The chromophore is in the distorted alpha-helix that runs along the axis of the can, close to the center of the can-like cylinder.

You can take a close look at the of GFP in the PDB entry 1ema.

The chromophore

1 Reference for the Structure
2 Related Structures and Topics
3 Notes and Literature References
4 Additional information on green fluorescent protein

Reference for the Structure

Crystal structure of the Aequorea victoria green fluorescent protein., Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075

Notes and Literature References

↑ Crystal structure of the Aequorea victoria green fluorescent protein., Ormö M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science 1996 6;273(5280):1392-1395. PMID:8703075
↑ The molecular structure of green fluorescent protein., Yang F, Moss LG, Phillips GN Jr, Nat Biotechnol. 1996 Oct;14(10):1246-51. PMID:9631087

Additional information on green fluorescent protein

Roger Y. Tsien (1998) The Green Fluorescent Protein. Annual Review of Biochemistry 67, 509-544.
[The GFP site] by Marc Zimmer, Ph. D., at Connecticut College, who authored [Glowing Genes: A Revolution In Biotechnology].