1uha
From Proteopedia
|
Crystal Structure of Pokeweed Lectin-D2
Overview
Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing, saccharides, stimulates peripheral lymphocytes to undergo mitosis by, binding to their cell surfaces. Four types of lectins have been isolated, from the roots of pokeweed (Phytolacca americana) and shown to contain, homologous domains but to have different molecular sizes and biological, properties. PL-D, the smallest lectin in the group, has two isolectins, PL-D1 and PL-D2. PL-D1 consists of 84 amino-acid residues, while PL-D2 is, identical to PL-D1 in sequence except for the lack of two C-terminal, residues, Leu83 and Thr84. The crystal structures of PL-D1 and PL-D2 were, solved by the molecular-replacement method and refined to 1.65 and 1.5 A, resolution with R factors of 17.2 and 17.6%, respectively. The PL-Ds are, composed of two repetitive chitin-binding domains, each of which has four, S-S bridges and one putative carbohydrate-binding site. The two, carbohydrate-binding sites in PL-D are located on one side of the, molecule. The relative orientation of the two domains in PL-D1 differs, from that in PL-D2. Two C-terminal residues of PL-D1 are invisible in the, present crystal structure, indicating the flexibility of the region. PL-D2, has a Ca2+ ion bound to the C-terminus on the molecular surface. A wide, distribution of acidic residues is characteristically observed on one side, of the C-terminal region of PL-D.
About this Structure
1UHA is a Single protein structure of sequence from Phytolacca americana with CA as ligand. Full crystallographic information is available from OCA.
Reference
Structures of two lectins from the roots of pokeweed (Phytolacca americana)., Fujii T, Hayashida M, Hamasu M, Ishiguro M, Hata Y, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):665-73. Epub 2004, Mar 23. PMID:15039554
Page seeded by OCA on Wed Nov 21 04:07:30 2007
