This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ulw
From Proteopedia
|
Crystal structure of P450nor Ser73Gly/Ser75Gly mutant
Overview
Nitric oxide reductase cytochrome P450nor catalyzes an unusual reaction, direct electron transfer from NAD(P)H to bound heme. Here, we succeeded in, determining the crystal structure of P450nor in a complex with an NADH, analogue, nicotinic acid adenine dinucleotide, which provides conclusive, evidence for the mechanism of the unprecedented electron transfer., Comparison of the structure with those of dinucleotide-free forms revealed, a global conformational change accompanied by intriguing local movements, caused by the binding of the pyridine nucleotide. Arg64 and Arg174 fix the, pyrophosphate moiety upon the dinucleotide binding. Stereo-selective, hydride transfer from NADH to NO-bound heme was suggested from the, structure, the nicotinic acid ring being fixed near the heme by the, conserved Thr residue in the I-helix and the upward-shifted propionate, side-chain of the heme. A proton channel near the NADH channel is formed, upon the dinucleotide binding, which should direct continuous transfer of, the hydride and proton. A salt-bridge network (Glu71-Arg64-Asp88) was, shown to be crucial for a high catalytic turnover.
About this Structure
1ULW is a Single protein structure of sequence from Fusarium oxysporum with HEM as ligand. Active as Nitric-oxide reductase, with EC number 1.7.99.7 Full crystallographic information is available from OCA.
Reference
Structural evidence for direct hydride transfer from NADH to cytochrome P450nor., Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H, J Mol Biol. 2004 Sep 3;342(1):207-17. PMID:15313618
Page seeded by OCA on Wed Nov 21 04:13:59 2007
