This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ogz

From Proteopedia

Revision as of 19:10, 29 October 2007 by OCA (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Image:1ogz.gif

1ogz, resolution 2.3Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANTS P39A COMPLEXED WITH EQUILENIN FROM PSEUDOMONAS TESTOSTERONI

Overview

KSI (ketosteroid isomerase) from Comamonas testosteroni is a homodimeric, enzyme that catalyses the allylic isomerization of Delta5-3-ketosteroids, to their conjugated Delta4-isomers at a reaction rate equivalent to the, diffusion-controlled limit. Based on the structural analysis of KSI at a, high resolution, the conserved cis-Pro39 residue was proposed to be, involved in the proper positioning of Asp38, a critical catalytic residue, since the residue was found not only to be structurally associated with, Asp38, but also to confer a structural rigidity on the local active-site, geometry consisting of Asp38, Pro39, Val40, Gly41 and Ser42 at the, flexible loop between b-strands B1 and B2. In order to investigate the, structural role of the conserved cis-Pro39 residue near the active site ... [(full description)]

About this Structure

1OGZ is a [Single protein] structure of sequence from [Comamonas testosteroni] with EQU as [ligand]. Active as [[1]], with EC number [5.3.3.1]. Full crystallographic information is available from [OCA].

Reference

The conserved cis-Pro39 residue plays a crucial role in the proper positioning of the catalytic base Asp38 in ketosteroid isomerase from Comamonas testosteroni., Nam GH, Cha SS, Yun YS, Oh YH, Hong BH, Lee HS, Choi KY, Biochem J. 2003 Oct 15;375(Pt 2):297-305. PMID:12852789

Page seeded by OCA on Mon Oct 29 21:15:07 2007

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ogz"
Personal tools