1ogz

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spinqualitylabelsall models 1ogz, resolution 2.3Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANTS P39A COMPLEXED WITH EQUILENIN FROM PSEUDOMONAS TESTOSTERONI

Overview

KSI (ketosteroid isomerase) from Comamonas testosteroni is a homodimeric, enzyme that catalyses the allylic isomerization of Delta5-3-ketosteroids, to their conjugated Delta4-isomers at a reaction rate equivalent to the, diffusion-controlled limit. Based on the structural analysis of KSI at a, high resolution, the conserved cis-Pro39 residue was proposed to be, involved in the proper positioning of Asp38, a critical catalytic residue, since the residue was found not only to be structurally associated with, Asp38, but also to confer a structural rigidity on the local active-site, geometry consisting of Asp38, Pro39, Val40, Gly41 and Ser42 at the, flexible loop between b-strands B1 and B2. In order to investigate the, structural role of the conserved cis-Pro39 residue near the active site ... [(full description)]

About this Structure

1OGZ is a [Single protein] structure of sequence from [Comamonas testosteroni] with EQU as [ligand]. Active as [[1]], with EC number [5.3.3.1]. Full crystallographic information is available from [OCA].

Reference

The conserved cis-Pro39 residue plays a crucial role in the proper positioning of the catalytic base Asp38 in ketosteroid isomerase from Comamonas testosteroni., Nam GH, Cha SS, Yun YS, Oh YH, Hong BH, Lee HS, Choi KY, Biochem J. 2003 Oct 15;375(Pt 2):297-305. PMID:12852789

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