1v6p
From Proteopedia
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Crystal structure of Cobrotoxin
Overview
By using single wavelength anomalous diffraction phasing based on the, anomalous signal from copper atoms, the crystal structure of atratoxin was, determined at the resolution of 1.5 A and was refined to an ultrahigh, resolution of 0.87 A. The ultrahigh resolution electron density maps, allowed the modeling of 38 amino acid residues in alternate conformations, and the location of 322 of 870 possible hydrogen atoms. To get accurate, information at the atomic level, atratoxin-b (an analog of atratoxin with, reduced toxicity) was also refined to an atomic resolution of 0.92 A. By, the sequence and structural comparison of these two atratoxins, Arg(33), and Arg(36) were identified to be critical to their varied toxicity. The, effect of copper ions on the distribution of hydrogen atoms in atratoxin, was discussed, and the interactions between copper ions and protein, residues were analyzed based on a statistical method, revealing a novel, pentahedral copper-binding motif.
About this Structure
1V6P is a Single protein structure of sequence from Naja atra with CU, CL, NA and EOH as ligands. Full crystallographic information is available from OCA.
Reference
The atomic resolution crystal structure of atratoxin determined by single wavelength anomalous diffraction phasing., Lou X, Liu Q, Tu X, Wang J, Teng M, Niu L, Schuller DJ, Huang Q, Hao Q, J Biol Chem. 2004 Sep 10;279(37):39094-104. Epub 2004 Jul 12. PMID:15252034
Page seeded by OCA on Wed Nov 21 04:29:00 2007
Categories: Naja atra | Single protein | Hao, Q. | Huang, Q. | Liu, Q. | Lou, X. | Niu, L. | Teng, M. | Tu, X. | Wang, J. | CL | CU | EOH | NA | Atomic resolution | Copper ion | Short-chain neurotoxin
