1v77
From Proteopedia
|
Crystal structure of the PH1877 protein
Overview
Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the, processing of pre-tRNA. Protein Ph1877p is one of essential components of, the hyperthermophilic archaeon Pyrococcus horikoshii OT3 RNase P [Biochem., Biophys. Res. Commun. 306 (2003) 666]. The crystal structure of Ph1877p, was determined at 1.8A by X-ray crystallography and refined to a, crystallographic R factor of 22.96% (Rfree of 26.77%). Ph1877p forms a TIM, barrel structure, consisting of ten alpha-helices and seven beta-strands, and has the closest similarity to the TIM barrel domain of Escherichia, coli cytosine deaminase with a root-mean square deviation of 3.0A. The, protein Ph1877p forms an oblate ellipsoid, approximate dimensions being, 45Ax43Ax39A, and the electrostatic representation indicated the presence, of several clusters of positively charged amino acids present on the, molecular surface. We made use of site-directed mutagenesis to assess the, role of twelve charged amino acids, Lys42, Arg68, Arg87, Arg90, Asp98, Arg107, His114, Lys123, Lys158, Arg176, Asp180, and Lys196 related to the, RNase P activity. Individual mutations of Arg90, Arg107, Lys123, Arg176, and Lys196 by Ala resulted in reconstituted particles with reduced, enzymatic activities (32-48%) as compared with that reconstituted RNase P, by wild-type Ph1877p. The results presented here provide an initial step, for definite understanding of how archaeal and eukaryotic RNase Ps mediate, substrate recognition and process 5'-leader sequence of pre-tRNA.
About this Structure
1V77 is a Single protein structure of sequence from Pyrococcus horikoshii. Active as Ribonuclease P, with EC number 3.1.26.5 Full crystallographic information is available from OCA.
Reference
Crystal structure of the ribonuclease P protein Ph1877p from hyperthermophilic archaeon Pyrococcus horikoshii OT3., Takagi H, Watanabe M, Kakuta Y, Kamachi R, Numata T, Tanaka I, Kimura M, Biochem Biophys Res Commun. 2004 Jul 2;319(3):787-94. PMID:15184052
Page seeded by OCA on Wed Nov 21 04:29:26 2007
