1vjm
From Proteopedia
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Deformation of helix C in the low-temperature L-intermediate of bacteriorhodopsin
Overview
X-ray and electron diffraction studies of specific reaction intermediates, or reaction intermediate analogues, have produced a consistent picture of, the structural mechanism of light-driven proton pumping by, bacteriorhodopsin. Of central importance within this picture is the, structure of the L-intermediate, which follows the retinal all-trans to, 13-cis photoisomerization step of the K-intermediate and sets the stage, for the primary proton transfer event from the positively charged Schiff, base to the negatively charged Asp-85. Here we report the structural, changes in bacteriorhodopsin following red light illumination at 150 K., Single crystal microspectrophotometry showed that only the L-intermediate, is populated in three-dimensional crystals under these conditions. The, experimental difference Fourier electron density map and refined, crystallographic structure were consistent with those previously presented, (Royant, A., Edman, K., Ursby, T., Pebay-Peyroula, E., Landau, E. M., and, Neutze, R. (2000) Nature 406, 645-648; Royant, A., Edman, K., Ursby, T., Pebay-Peyroula, E., Landau, E. M., and Neutze, R. (2001) Photochem., Photobiol. 74, 794-804). Based on the refined crystallographic structures, molecular dynamic simulations were used to examine the influence of the, conformational change of the protein that is associated with the K-to-L, transition on retinal dynamics. Implications regarding the structural, mechanism for proton pumping by bacteriorhodopsin are discussed.
About this Structure
1VJM is a Single protein structure of sequence from Halobacterium sp. with RET as ligand. This structure superseeds the now removed PDB entry 1R3P. Full crystallographic information is available from OCA.
Reference
Deformation of helix C in the low temperature L-intermediate of bacteriorhodopsin., Edman K, Royant A, Larsson G, Jacobson F, Taylor T, van der Spoel D, Landau EM, Pebay-Peyroula E, Neutze R, J Biol Chem. 2004 Jan 16;279(3):2147-58. Epub 2003 Oct 7. PMID:14532280
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