1vpt
From Proteopedia
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AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE
Overview
VP39 is a bifunctional vaccinia virus protein that acts as both an mRNA, cap-specific RNA 2'-O-methyltransferase and a poly(A) polymerase, processivity factor. Here, we report the 1.85 A crystal structure of a, VP39 variant complexed with its AdoMet cofactor. VP39 comprises a single, core domain with structural similarity to the catalytic domains of other, methyltransferases. Surface features and mutagenesis data suggest two, possible RNA-binding sites with novel underlying architecture, one of, which forms a cleft spanning the region adjacent to the methyltransferase, active site. This report provides a prototypic structure for an RNA, methyltransferase, a protein that interacts with the mRNA 5' cap, and an, intact poxvirus protein.
About this Structure
1VPT is a Single protein structure of sequence from Vaccinia virus with SAM as ligand. Active as Polynucleotide adenylyltransferase, with EC number 2.7.7.19 Full crystallographic information is available from OCA.
Reference
The 1.85 A structure of vaccinia protein VP39: a bifunctional enzyme that participates in the modification of both mRNA ends., Hodel AE, Gershon PD, Shi X, Quiocho FA, Cell. 1996 Apr 19;85(2):247-56. PMID:8612277
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