1vpw
From Proteopedia
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STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA
Overview
The crystal structure of the purine repressor mutant L54M bound to, hypoxanthine and to the purF operator provides a stereochemical, understanding of the high DNA affinity of this hinge helix mutant., Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA, complex reveals that these purine repressors bind and kink DNA similarly, despite significant differences in their minor groove contacts and routes, to interdigitation of the central C.G:G.C base pair step. Modeling, studies, supported by genetic and biochemical data, show that the, stereochemistry of the backbone atoms of the abutting hinge helices, combined with the rigidity of the kinked base pair step constrain the, interdigitating residue to leucine or methionine for the LacI/GalR family, of transcription regulators.
About this Structure
1VPW is a Single protein structure of sequence from Escherichia coli with HPA as ligand. Full crystallographic information is available from OCA.
Reference
The structure of PurR mutant L54M shows an alternative route to DNA kinking., Arvidson DN, Lu F, Faber C, Zalkin H, Brennan RG, Nat Struct Biol. 1998 Jun;5(6):436-41. PMID:9628480
Page seeded by OCA on Wed Nov 21 05:00:00 2007
