1wav

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spinqualitylabelsall models 1wav, resolution 2.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN

Overview

The form-B monoclinic insulin crystal was obtained from the sodium citrate, buffer with 1% zinc chloride, keeping phenolic content between 0.76% and, 1.25%. Its space group is P2(1), cell constants are: a = 4.924 nm, b =, 6.094 nm, c = 4.818 nm, beta = 95.8 degrees. There are 6 insulin molecules, which form a hexamer. The initial phase was obtained by using rotation, function program of X-PLOR program package and molecular packing program, of our laboratory. The molecular model was chosen from 4 zinc bovine, insulin hexamer. After the preliminary refinement by using the, macromolecular rigid body refinement technique, the molecular model was, further refined and adjusted by using the energy-minimizing, stereochemically restrained least-squared refinement on the difference, Fourier maps. The final R-factor is 22.4% at 0.3 nm resolution, the r.m.s., deviations from standard bond length and bond angle are 0.0022 nm and 4.7, degrees, respectively.

About this Structure

1WAV is a Protein complex structure of sequences from Sus scrofa with ZN and IPH as ligands. Full crystallographic information is available from OCA.

Reference

Molecular replacement study on form-B monoclinic crystal of insulin., Ding J, Wan Z, Chang W, Liang D, Sci China C Life Sci. 1996 Apr;39(2):144-53. PMID:8760462

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