1w8p

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spinqualitylabelsall models 1w8p, resolution 2.08Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURAL PROPERTIES OF THE B25TYR-NME-B26PHE INSULIN MUTANT.

Overview

The origins of differentiation of insulin from insulin-like growth factor, I (IGF-I) are still unknown. To address the problem of a structural and, biological switch from the mostly metabolic hormonal activity of insulin, to the predominant growth factor activities of IGF-I, an insulin analogue, with IGF-I-like structural features has been synthesized. Insulin residues, Phe(B25) and Tyr(B26) have been swapped with the IGF-I-like Tyr(24) and, Phe(25) sequence with a simultaneous methylation of the peptide nitrogen, of residue Phe(B26). These modifications were expected to introduce a, substantial kink in the main chain, as observed at residue Phe(25) in the, IGF-I crystal structure. These alterations should provide insight into the, structural origins of insulin-IGF-I structural and ... [(full description)]

About this Structure

1W8P is a [Protein complex] structure of sequences from [Homo sapiens] with ZN and IPH as [ligands]. Full crystallographic information is available from [OCA].

Reference

Toward the insulin-IGF-I intermediate structures: functional and structural properties of the [TyrB25NMePheB26] insulin mutant., Zakova L, Brynda J, Au-Alvarez O, Dodson EJ, Dodson GG, Whittingham JL, Brzozowski AM, Biochemistry. 2004 Dec 28;43(51):16293-300. PMID:15610023

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