2cbz
From Proteopedia
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STRUCTURE OF THE HUMAN MULTIDRUG RESISTANCE PROTEIN 1 NUCLEOTIDE BINDING DOMAIN 1
Overview
Human multidrug resistance protein 1 (MRP1) is a membrane protein that, belongs to the ATP-binding cassette (ABC) superfamily of transport, proteins. MRP1 contributes to chemotherapy failure by exporting a wide, range of anti-cancer drugs when over expressed in the plasma membrane of, cells. Here, we report the first high-resolution crystal structure of, human MRP1-NBD1. Drug efflux requires energy resulting from hydrolysis of, ATP by nucleotide binding domains (NBDs). Contrary to the prokaryotic, NBDs, the extremely low intrinsic ATPase activity of isolated MRP1-NBDs, allowed us to obtain the structure of wild-type NBD1 in complex with, Mg2+/ATP. The structure shows that MRP1-NBD1 adopts a canonical fold, but, reveals an unexpected non-productive conformation of the catalytic site, ... [(full description)]
About this Structure
2CBZ is a [Single protein] structure of sequence from [Homo sapiens] with MG and ATP as [ligands]. Full crystallographic information is available from [OCA].
Reference
Structure of the human multidrug resistance protein 1 nucleotide binding domain 1 bound to Mg2+/ATP reveals a non-productive catalytic site., Ramaen O, Leulliot N, Sizun C, Ulryck N, Pamlard O, Lallemand JY, Tilbeurgh H, Jacquet E, J Mol Biol. 2006 Jun 16;359(4):940-9. Epub 2006 May 2. PMID:16697012
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