1wzu
From Proteopedia
|
Crystal structure of quinolinate synthase (nadA)
Overview
A gene encoding a quinolinate synthase has been identified in the, hyperthermophilic archaeon Pyrococcus horikoshii via genome sequencing., The gene was overexpressed in Escherichia coli, and the crystal structure, of the produced enzyme was determined to 2.0 A resolution in the presence, of malate, a substrate analogue. The overall structure exhibits a unique, triangular architecture composed of a 3-fold repeat of three-layer, (alphabetaalpha) sandwich folding. Although some aspects of the fold, homologous to the each domain have been observed previously, the overall, structure of quinolinate synthase shows no similarity to any known protein, structure. The three analogous domains are related to a pseudo-3-fold, symmetry. The active site is located at the interface of the three domains, and is centered on the pseudo-3-fold axis. The malate molecule is tightly, held near the bottom of the active site cavity. The model of the catalytic, state during the first condensation step of the quinolinate synthase, reaction indicates that the elimination of inorganic phosphate from, dihydroxyacetone phosphate may precede the condensation reaction.
About this Structure
1WZU is a Single protein structure of sequence from Pyrococcus horikoshii with MLT as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the NAD biosynthetic enzyme quinolinate synthase., Sakuraba H, Tsuge H, Yoneda K, Katunuma N, Ohshima T, J Biol Chem. 2005 Jul 22;280(29):26645-8. Epub 2005 Jun 3. PMID:15937336
Page seeded by OCA on Wed Nov 21 05:46:03 2007
