1xji

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Bacteriorhodopsin crystallized in bicelles at room temperature

Overview

We showed previously that high-quality crystals of bacteriorhodopsin (bR), from Halobacterium salinarum can be obtained from bicelle-forming, DMPC/CHAPSO mixtures at 37 degrees C. As many membrane proteins are not, sufficiently stable for crystallization at this high temperature, we, tested whether the bicelle method could be applied at a lower temperature., Here we show that bR can be crystallized at room temperature using two, different bicelle-forming compositions: DMPC/CHAPSO and DTPC/CHAPSO. The, DTPC/CHAPSO crystals grown at room temperature are essentially identical, to the previous, twinned crystals: space group P21 with unit cell, dimensions of a = 44.7 A, b = 108.7 A, c = 55.8 A, beta = 113.6 degrees ., The room-temperature DMPC/CHAPSO crystals are untwinned, however, and, belong to space group C222(1) with the following unit cell dimensions: a =, 44.7 A, b = 102.5 A, c = 128.2 A. The bR protein packs into almost, identical layers in the two crystal forms, but the layers stack, differently. The new untwinned crystal form yielded clear density for a, previously unresolved CHAPSO molecule inserted between protein subunits, within the layers. The ability to grow crystals at room temperature, significantly expands the applicability of bicelle crystallization.

About this Structure

1XJI is a Single protein structure of sequence from Halobacterium salinarum with RET, D12, D10, C14, OCT and CPS as ligands. Full crystallographic information is available from OCA.

Reference

Crystallization of bacteriorhodopsin from bicelle formulations at room temperature., Faham S, Boulting GL, Massey EA, Yohannan S, Yang D, Bowie JU, Protein Sci. 2005 Mar;14(3):836-40. Epub 2005 Feb 2. PMID:15689517

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