2bkb

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spinqualitylabelsall models 2bkb, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Q69E-FESOD

Overview

Fe-containing superoxide dismutase's active site Fe is coordinated by a, solvent molecule, whose protonation state is coupled to the Fe oxidation, state. Thus, we have proposed that H-bonding between glutamine 69 and this, solvent molecule can strongly influence the redox activity of the Fe in, superoxide dismutase (SOD). We show here that mutation of this Gln to His, subtly alters the active site structure but preserves 30% activity. In, contrast, mutation to Glu otherwise preserves the active site structure, but inactivates the enzyme. Thus, enzyme function correlates not with atom, positions but with residue identity (chemistry), in this case. We observe, strong destabilization of the Q69E-FeSOD oxidized state relative to the, reduced state and intermediate destabilization of oxidized ... [(full description)]

About this Structure

2BKB is a [Single protein] structure of sequence from [Escherichia coli] with FE2 as [ligand]. Active as [[1]], with EC number [1.15.1.1]. Full crystallographic information is available from [OCA].

Reference

The crucial importance of chemistry in the structure-function link: manipulating hydrogen bonding in iron-containing superoxide dismutase., Yikilmaz E, Rodgers DW, Miller AF, Biochemistry. 2006 Jan 31;45(4):1151-61. PMID:16430211

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