1z4o
From Proteopedia
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Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate
Overview
This communication reports the X-ray crystal structure of the -D-galactose-1-phosphate complex with that of Lactococcus lactis -phosphoglucomutase (-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of -PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the -glucose 1,6-bisphosphate ligand and rules out the proposal of a -PGM-glucose-6-phosphate-1-MgF3- complex.
About this Structure
1Z4O is a Single protein structure of sequence from Lactococcus lactis with MG and GL1 as ligands. Active as Beta-phosphoglucomutase, with EC number 5.4.2.6 Full crystallographic information is available from OCA.
Reference
Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase., Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN, J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149
Page seeded by OCA on Wed Nov 21 07:14:03 2007
