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1z8s
From Proteopedia
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DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)
Overview
The helicase-primase interaction is a critical event in DNA replication, and is mediated by a putative helicase-interaction domain within the, primase. The solution structure of the helicase-interaction domain of DnaG, reveals that it is made up of two independent subdomains: an N-terminal, six-helix module and a C-terminal two-helix module that contains the, residues of the primase previously identified as important in the, interaction with the helicase. We show that the two-helix module alone is, sufficient for strong binding between the primase and the helicase but, fails to activate the helicase; both subdomains are required for helicase, activation. The six-helix module of the primase has only one close, structural homolog, the N-terminal domain of the corresponding helicase., This surprising structural relationship, coupled with the differences in, surface properties of the two molecules, suggests how the, helicase-interaction domain may perturb the structure of the helicase and, lead to activation.
About this Structure
1Z8S is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation., Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP, Structure. 2005 Apr;13(4):609-16. PMID:15837199
Page seeded by OCA on Wed Nov 21 07:18:20 2007
