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1zfd
From Proteopedia
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SWI5 ZINC FINGER DOMAIN 2, NMR, 45 STRUCTURES
Overview
This paper describes the detailed three-dimensional structures of two, zinc-finger domains from the yeast transcription factor SWI5, calculated, using the results of the n.m.r. experiments described in the accompanying, paper. The structure of finger 2 is essentially similar to those, previously obtained by others for isolated, synthetic single zinc-finger, domains in solution, and for the three zinc-finger peptide Zif268 in its, crystalline complex with DNA. The N-terminal half of the sequence forms a, two-stranded, irregular beta-sheet containing both of the metal-binding, cysteine residues, while the remainder of the structure forms a helix., Approximately the first half of this helix is alpha-helical, whereas the, C-terminal portion, including the two metal-binding histidine residues, is, 3(10) helical. Four invariant hydrophobic residues form a core to the, structure. In contrast to all previously described structures of, zinc-finger domains, finger 1 has an additional strand in the beta-sheet, formed by residues N-terminal to the formal start of the finger motif., This additional strand plays a role in stabilising the folded form of, finger 1, since a two-finger peptide lacking the N-terminal residues, showed folded structure in finger 2 but not in finger 1.
About this Structure
1ZFD is a Single protein structure of sequence from Saccharomyces cerevisiae with ZN as ligand. Full crystallographic information is available from OCA.
Reference
Solution structures of two zinc-finger domains from SWI5 obtained using two-dimensional 1H nuclear magnetic resonance spectroscopy. A zinc-finger structure with a third strand of beta-sheet., Neuhaus D, Nakaseko Y, Schwabe JW, Klug A, J Mol Biol. 1992 Nov 20;228(2):637-51. PMID:1453468
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