1zno

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spinqualitylabelsall models 1zno, resolution 2.Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of VC702 from Vibrio Cholerae, Northeast Structural Genomics Consortium Target: VcP1

Overview

VC0702, a conserved hypothetical protein of unknown function from Vibrio, cholerae, resides in a three-gene operon containing the MbaA gene that, encodes for a GGDEF and EAL domain-containing protein which is involved in, regulating formation of the extracellular matrix of biofilms in Vibrio, cholerae. The VC0702 crystal structure has been determined at 2.0 A and, refined to Rwork = 22.8% and Rfree = 26.3%. VC0702 crystallized in an, orthorhombic crystal lattice in the C222(1) space group with dimensions of, a = 66.61 A, b = 88.118 A, and c = 118.35 A with a homodimer in the, asymmetric unit. VC0702, which forms a mixed alpha + beta three-layered, alphabetaalpha sandwich, belongs to the Pfam DUF84 and COG1986 families of, proteins. Sequence conservation within the DUF84 and COG1986 families was, used to identify a conserved patch of surface residues that define a cleft, and potential substrate-binding site in VC0702. The three-dimensional, structure of VC0702 is similar to that of Mj0226 from Methanococcus, janeschii, which has been identified as a novel NTPase that binds NTP in a, deep cleft similarly located to the conserved patch of surface residues, that define an analogous cleft in VC0702. Collectively, the data suggest, that VC0702 may have a biochemical function that involves NTP binding and, phosphatase activity of some kind, and is likely involved in regulation of, the signaling pathway that controls biofilm formation and maintenance in, Vibrio cholerae.

About this Structure

1ZNO is a Single protein structure of sequence from Vibrio cholerae with MG as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of VC0702 at 2.0 A: conserved hypothetical protein from Vibrio cholerae., Ni S, Forouhar F, Bussiere DE, Robinson H, Kennedy MA, Proteins. 2006 Jun 1;63(4):733-41. PMID:16498616

Page seeded by OCA on Wed Nov 21 07:33:31 2007