2anb

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spinqualitylabelsall models 2anb, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure Of Oligomeric E.coli Guanylate Kinase In Complex With GMP

Overview

Guanosine monophosphate kinases (GMPKs), which catalyze the, phosphorylation of GMP and dGMP to their diphosphate form, have been, characterized as monomeric enzymes in eukaryotes and prokaryotes. Here, we, report that GMPK from Escherichia coli (ecGMPK) assembles in solution and, in the crystal as several different oligomers. Thermodynamic analysis of, ecGMPK using differential scanning calorimetry shows that the enzyme is in, equilibrium between a dimer and higher order oligomers, whose relative, amounts depend on protein concentration, ionic strength, and the presence, of ATP. Crystallographic structures of ecGMPK in the apo, GMP and, GDP-bound forms were solved at 3.2A, 2.9A and 2.4A resolution, respectively. ecGMPK forms a hexamer with D3 symmetry in all crystal, forms, in which the two nucleotide-binding domains are able to undergo, closure comparable to that of monomeric GMPKs. The 2-fold and 3-fold, interfaces involve a 20-residue C-terminal extension and a sequence, signature, respectively, that are missing from monomeric eukaryotic GMPKs, explaining why ecGMPK forms oligomers. These signatures are found in GMPKs, from proteobacteria, some of which are human pathogens. GMPKs from these, bacteria are thus likely to form the same quaternary structures. The shift, of the thermodynamic equilibrium towards the dimer at low ecGMPK, concentration together with the observation that inter-subunit, interactions partially occlude the ATP-binding site in the hexameric, structure suggest that the dimer may be the active species at, physiological enzyme concentration.

About this Structure

2ANB is a Single protein structure of sequence from Escherichia coli with SO4 and 5GP as ligands. Active as Guanylate kinase, with EC number 2.7.4.8 Full crystallographic information is available from OCA.

Reference

Calorimetric and crystallographic analysis of the oligomeric structure of Escherichia coli GMP kinase., Hible G, Renault L, Schaeffer F, Christova P, Zoe Radulescu A, Evrin C, Gilles AM, Cherfils J, J Mol Biol. 2005 Oct 7;352(5):1044-59. PMID:16140325

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