2b64
From Proteopedia
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30S ribosomal subunit, tRNAs, mRNA and release factor RF1 from a crystal structure of the whole ribosomal complex. This file contains the 30S subunit, tRNAs, mRNA and release factor RF1 from a crystal structure of the whole ribosomal complex". The entire crystal structure contains one 70S ribosome, tRNAs, mRNA and release factor RF1 and is described in remark 400.
Overview
During protein synthesis, translational release factors catalyze the, release of the polypeptide chain when a stop codon on the mRNA reaches the, A site of the ribosome. The detailed mechanism of this process is, currently unknown. We present here the crystal structures of the ribosome, from Thermus thermophilus with RF1 and RF2 bound to their cognate stop, codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The, structures reveal details of interactions of the factors with the ribosome, and mRNA, including elements previously implicated in decoding and peptide, release. They also shed light on conformational changes both in the, factors and in the ribosome during termination. Differences seen in the, interaction of RF1 and RF2 with the L11 region of the ribosome allow us to, rationalize previous biochemical data. Finally, this work demonstrates the, feasibility of crystallizing ribosomes with bound factors at a defined, state along the translational pathway.
About this Structure
2B64 is a Protein complex structure of sequences from Escherichia coli, Thermus thermophilus and Thermus thermophilus hb27. Full crystallographic information is available from OCA.
Reference
Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon., Petry S, Brodersen DE, Murphy FV 4th, Dunham CM, Selmer M, Tarry MJ, Kelley AC, Ramakrishnan V, Cell. 2005 Dec 29;123(7):1255-66. PMID:16377566
Page seeded by OCA on Wed Nov 21 08:35:23 2007
