2bcw
From Proteopedia
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Coordinates of the N-terminal domain of ribosomal protein L11,C-terminal domain of ribosomal protein L7/L12 and a portion of the G' domain of elongation factor G, as fitted into cryo-em map of an Escherichia coli 70S*EF-G*GDP*fusidic acid complex
Overview
During tRNA translocation on the ribosome, an arc-like connection (ALC) is, formed between the G' domain of elongation factor G (EF-G) and the, L7/L12-stalk base of the large ribosomal subunit in the GDP state. To, delineate the boundary of EF-G within the ALC, we tagged an amino acid, residue near the tip of the G' domain of EF-G with undecagold, which was, then visualized with three-dimensional cryo-electron microscopy (cryo-EM)., Two distinct positions for the undecagold, observed in the GTP-state and, GDP-state cryo-EM maps of the ribosome bound EF-G, allowed us to determine, the movement of the labeled amino acid. Molecular analyses of the cryo-EM, maps show: (1) that three structural components, the N-terminal domain of, ribosomal protein L11, the C-terminal domain of ribosomal protein L7/L12, and the G' domain of EF-G, participate in formation of the ALC; and (2), that both EF-G and the ribosomal protein L7/L12 undergo large, conformational changes to form the ALC.
About this Structure
2BCW is a Protein complex structure of sequences from Escherichia coli, Thermotoga maritima and Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM., Datta PP, Sharma MR, Qi L, Frank J, Agrawal RK, Mol Cell. 2005 Dec 9;20(5):723-31. PMID:16337596
Page seeded by OCA on Wed Nov 21 08:44:00 2007
