2bgh

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spinqualitylabelsall models 2bgh, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF VINORINE SYNTHASE

Overview

Vinorine synthase is an acetyltransferase that occupies a central role in, the biosynthesis of the antiarrhythmic monoterpenoid indole alkaloid, ajmaline in the plant Rauvolfia. Vinorine synthase belongs to the, benzylalcohol acetyl-, anthocyanin-O-hydroxy-cinnamoyl-, anthranilate-N-hydroxy-cinnamoyl/benzoyl-, deacetylvindoline, acetyltransferase (BAHD) enzyme superfamily, members of which are involved, in the biosynthesis of several important drugs, such as morphine, Taxol, or vindoline, a precursor of the anti-cancer drugs vincaleucoblastine and, vincristine. The x-ray structure of vinorine synthase is described at, 2.6-angstrom resolution. Despite low sequence identity, the two-domain, structure of vinorine synthase shows surprising similarity with structures, of several CoA-dependent acyltransferases such as dihydrolipoyl, transacetylase, polyketide-associated protein A5, and carnitine, acetyltransferase. All conserved residues typical for the BAHD family are, found in domain 1. His160 of the HXXXD motif functions as a general base, during catalysis. It is located in the center of the reaction channel at, the interface of both domains and is accessible from both sides. The, channel runs through the entire molecule, allowing the substrate and, co-substrate to bind independently. Asp164 points away from the catalytic, site and seems to be of structural rather than catalytic importance., Surprisingly, the DFGWG motif, which is indispensable for the catalyzed, reaction and unique to the BAHD family, is located far away from the, active site and seems to play only a structural role. Vinorine synthase, represents the first solved protein structure of the BAHD superfamily.

About this Structure

2BGH is a Single protein structure of sequence from Rauvolfia serpentina. Active as Vinorine synthase, with EC number 2.3.1.160 Full crystallographic information is available from OCA.

Reference

Crystal structure of vinorine synthase, the first representative of the BAHD superfamily., Ma X, Koepke J, Panjikar S, Fritzsch G, Stockigt J, J Biol Chem. 2005 Apr 8;280(14):13576-83. Epub 2005 Jan 22. PMID:15665331

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