2biu
From Proteopedia
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CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION, DMSO COMPLEX
Overview
In the pharmaceutical industry, knowledge of the three-dimensional, structure of a specific target facilitates the drug-discovery process., Despite possessing favoured analytical properties such as high purity and, monodispersion in light scattering, some proteins are not capable of, forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of, cyclophilin that is expressed in the mitochondria, was selected as a drug, target for the treatment of cardiac disorders. As the wild-type enzyme, defied all attempts at crystallization, protein engineering on the enzyme, surface was performed. The K133I mutant gave crystals that diffracted to, 1.7 A resolution using in-house X-ray facilities and were suitable for, soaking experiments. The crystals were very robust and diffraction ... [(full description)]
About this Structure
2BIU is a [Single protein] structure of sequence from [Homo sapiens] with DMS as [ligand]. Active as [[1]], with EC number [5.2.1.8]. Full crystallographic information is available from [OCA].
Reference
Crystal engineering yields crystals of cyclophilin D diffracting to 1.7 A resolution., Schlatter D, Thoma R, Kung E, Stihle M, Muller F, Borroni E, Cesura A, Hennig M, Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):513-9. Epub 2005, Apr 20. PMID:15858260
Page seeded by OCA on Mon Oct 29 21:48:53 2007
