2cjk
From Proteopedia
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STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA
Overview
The recognition of specific signals encoded within the 3'-untranslated, region of the newly transcribed mRNA triggers the assembly of a, multiprotein machine that modifies its 3'-end. Hrp1 recognises one of such, signals, the so-called polyadenylation enhancement element (PEE), promoting the recruitment of other polyadenylation factors in yeast. The, molecular bases of this interaction are revealed here by the solution, structure of a complex between Hrp1 and an oligonucleotide mimicking the, PEE. Six consecutive bases (AUAUAU) are specifically recognised by two, RNA-binding domains arranged in tandem. Both protein and RNA undergo, significant conformational changes upon complex formation with a, concomitant large surface burial of RNA bases. Key aspects of RNA, specificity can be explained by the presence of intermolecular, aromatic-aromatic contacts and hydrogen bonds. Altogether, the Hrp1-PEE, structure represents one of the first steps towards understanding of the, assembly of the cleavage and polyadenylation machinery at the atomic, level.
About this Structure
2CJK is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Grabbing the message: structural basis of mRNA 3'UTR recognition by Hrp1., Perez-Canadillas JM, EMBO J. 2006 Jul 12;25(13):3167-78. Epub 2006 Jun 22. PMID:16794580
Page seeded by OCA on Wed Nov 21 09:08:29 2007
