1w7z
From Proteopedia
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CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)
Overview
The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the, family of squash inhibitors and is one of the strongest inhibitors known, for trypsin. The eight independent molecules of EETI-II in the crystal, structure reported here provide a good opportunity to test the hypothesis, that this small cystine-knot protein (knottin) is sufficiently rigid to be, used as a molecular scaffold for protein-engineering purposes. To extend, this test, the structures of two complexes of EETI-II with trypsin have, also been determined, one carrying a four-amino-acid mutation of EETI-II., The remarkable similarity of these structures confirms the rigidity of the, molecular framework and hence its suitability as a molecular scaffold.
About this Structure
1W7Z is a [Single protein] structure of sequence from [Ecballium elaterium] with NA and FMT as [ligands]. Full crystallographic information is available from [OCA].
Reference
Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold., Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759
Page seeded by OCA on Mon Oct 29 21:51:02 2007
