1w7o

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1w7o, resolution 1.81Å

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CYTOCHROME C3 FROM DESULFOMICROBIUM BACULATUS

Overview

The tetraheme cytochrome c3 isolated from Desulfomicrobium baculatum (DSM, 1743)(Dsmb) was cloned, and the sequence analysis showed that this, cytochrome differs in just three amino acid residues from the cytochrome, c3 isolated from Desulfomicrobium norvegicum (Dsmn): (DsmnXXDsmb) Thr-37, --> Ser, Val-45 --> Ala, and Phe-88 --> Tyr. X-ray crystallography was, used to determine the structure of cytochrome c3 from Dsmb, showing that, it is very similar to the published structure of cytochrome c3 from Dsmn., A detailed thermodynamic and kinetic characterization of these two, tetraheme cytochromes c3 was performed by using NMR and visible, spectroscopy. The results obtained show that the network of, cooperativities between the redox and protonic centers is consistent with, a ... [(full description)]

About this Structure

1W7O is a [Single protein] structure of sequence from [Desulfomicrobium baculatum] with HEC as [ligand]. Full crystallographic information is available from [OCA].

Reference

Proton-assisted two-electron transfer in natural variants of tetraheme cytochromes from Desulfomicrobium Sp., Correia IJ, Paquete CM, Coelho A, Almeida CC, Catarino T, Louro RO, Frazao C, Saraiva LM, Carrondo MA, Turner DL, Xavier AV, J Biol Chem. 2004 Dec 10;279(50):52227-37. Epub 2004 Sep 28. PMID:15456779

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