1h9c
From Proteopedia
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NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.
Overview
The determination by NMR of the solution structure of the phosphorylated, enzyme IIB (P-IIB(Chb)) of the N,N'-diacetylchitobiose-specific, phosphoenolpyruvate-dependent phosphotransferase system of Escherichia, coli is presented. Most of the backbone and side-chain resonances were, assigned using a variety of mostly heteronuclear NMR experiments. The, remaining resonances were assigned with the help of the structure, calculations.NOE-derived distance restraints were used in distance, geometry calculations followed by molecular dynamics and simulated, annealing protocols. In addition, combinations of ambiguous restraints, were used to resolve ambiguities in the NOE assignments. By combining sets, of ambiguous and unambiguous restraints into new ambiguous restraints, an, error function was ... [(full description)]
About this Structure
1H9C is a [Single protein] structure of sequence from [Escherichia coli]. Active as [[1]], with EC number [2.7.1.69]. Full crystallographic information is available from [OCA].
Reference
NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli., Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM, J Mol Biol. 2001 May 18;308(5):993-1009. PMID:11352587
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