This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2du1
From Proteopedia
|
Crystal Structure of basic winged bean lectin in complex with Methyl-alpha-N-acetyl-D galactosamine
Overview
The crystal structure of the complexes of basic winged-bean lectin with, galactose, 2-methoxygalactose, N-acetylgalactosamine and, methyl-alpha-N-acetylgalactosamine have been determined. Lectin-sugar, interactions involve four hydrogen bonds and a stacking interaction in all, of the complexes. In addition, an N-H...O hydrogen bond involving the, hydroxyl group at C2 exists in the galactose and 2-methoxygalactose, complexes. An additional hydrophobic interaction involving the methyl, group in the latter leads to the higher affinity of the methyl derivative., In the lectin-N-acetylgalactosamine complex the N-H...O hydrogen bond is, lost, but a compensatory hydrogen bond is formed involving the O atom of, the acetamido group. In addition, the CH(3) moiety of the acetamido group, is involved in hydrophobic interactions. Consequently, the 2-methyl and, acetamido derivatives of galactose have nearly the same affinity for the, lectin. The methyl group alpha-linked to the galactose takes part in, additional hydrophobic interactions. Therefore, methyl-alpha-N-acetylgalactosamine has a higher affinity than, N-acetylgalactosamine for the lectin. The structures of basic winged-bean, lectin-sugar complexes provide a framework for examining the relative, affinity of galactose and galactosamine for the lectins that bind to them., The complexes also lead to a structural explanation for the blood-group, specificity of basic winged-bean lectin.
About this Structure
2DU1 is a Single protein structure of sequence from Psophocarpus tetragonolobus with MGC, NAG, CA and MN as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:17057334
Page seeded by OCA on Wed Nov 21 09:47:00 2007
Categories: Psophocarpus tetragonolobus | Single protein | Katiyar, S. | Kulkarni, K.A. | Suguna, K. | Surolia, A. | Vijayan, M. | CA | MGC | MN | NAG | Agglutinin | Glycosylated protein | Legume lectin
