2er0
From Proteopedia
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X-RAY STUDIES OF ASPARTIC PROTEINASE-STATINE INHIBITOR COMPLEXES
Overview
The conformation of a statine-containing renin inhibitor complexed with, the aspartic proteinase from the fungus Endothia parasitica (EC 3.4.23.6), has been determined by X-ray diffraction at 2.2-A resolution (R = 0.17)., We describe the structure of the complex at high resolution and compare, this with a 3.0-A resolution analysis of a bound inhibitor, L-364,099, containing a cyclohexylalanine analogue of statine. The inhibitors bind in, extended conformations in the long active-site cleft, and the hydroxyl of, the transition-state analogue, statine, interacts strongly with the, catalytic aspartates via hydrogen bonds to the essential carboxyl groups., This work provides a detailed structural analysis of the role of statine, in peptide inhibitors. It shows conclusively that statine should be, considered a dipeptide analogue (occupying P1 to P1') despite lacking the, equivalent of a P1' side chain, although other inhibitor residues, (especially P2) may compensate by interacting at the unoccupied S1', specificity subsite.
About this Structure
2ER0 is a Single protein structure of sequence from [1]. Active as Hydrolase, with EC number 3.4.23.18, 3.4.23.28 and 3.4.23.30 3.4.21.103, 3.4.23.18, 3.4.23.28 and 3.4.23.30 Full crystallographic information is available from OCA.
Reference
X-ray studies of aspartic proteinase-statine inhibitor complexes., Cooper JB, Foundling SI, Blundell TL, Boger J, Jupp RA, Kay J, Biochemistry. 1989 Oct 17;28(21):8596-603. PMID:2690945
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