2c8n
From Proteopedia
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THE STRUCTURE OF A FAMILY 51 ARABINOFURANOSIDASE, ARAF51, FROM CLOSTRIDIUM THERMOCELLUM IN COMPLEX WITH 1,3-LINKED ARABINOSIDE OF XYLOBIOSE.
Overview
The digestion of the plant cell wall requires the concerted action of a, diverse repertoire of enzyme activities. An important component of these, hydrolase consortia are arabinofuranosidases, which release, L-arabinofuranose moieties from a range of plant structural, polysaccharides. The anaerobic bacterium Clostridium thermocellum, a, highly efficient plant cell wall degrader, possesses a single, alpha-L-arabinofuranosidase (EC 3.2.1.55), CtAraf51A, located in GH51, (glycoside hydrolase family 51). The crystal structure of the enzyme has, been solved in native form and in 'Michaelis' complexes with both, alpha-1,5-linked arabinotriose and alpha-1,3 arabinoxylobiose, both, forming a hexamer in the asymmetric unit. Kinetic studies reveal that, CtAraf51A, in contrast with ... [(full description)]
About this Structure
2C8N is a [Single protein] structure of sequence from [Clostridium thermocellum] with EDO as [ligand]. Active as [[1]], with EC number [3.2.1.55]. Full crystallographic information is available from [OCA].
Reference
Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum., Taylor EJ, Smith NL, Turkenburg JP, D'Souza S, Gilbert HJ, Davies GJ, Biochem J. 2006 Apr 1;395(1):31-7. PMID:16336192
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