2f34

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spinqualitylabelsall models 2f34, resolution 1.74Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of the GluR5 Ligand Binding Core Dimer with UBP310 At 1.74 Angstroms Resolution

Overview

Glutamate receptor (GluR) ion channels mediate fast synaptic transmission, in the mammalian CNS. Numerous crystallographic studies, the majority on, the GluR2-subtype AMPA receptor, have revealed the structural basis for, binding of subtype-specific agonists. In contrast, because there are far, fewer antagonist-bound structures, the mechanisms for antagonist binding, are much less well understood, particularly for kainate receptors that, exist as multiple subtypes with a distinct biology encoded by the GluR5-7, KA1, and KA2 genes. We describe here high-resolution crystal structures, for the GluR5 ligand-binding core complex with UBP302 and UBP310, novel, GluR5-selective antagonists. The crystal structures reveal the structural, basis for the high selectivity for GluR5 observed in radiolabel, displacement assays for the isolated ligand binding cores of the GluR2, GluR5, and GluR6 subunits and during inhibition of glutamate-activated, currents in studies on full-length ion channels. The antagonists bind via, a novel mechanism and do not form direct contacts with the E723 side chain, as occurs in all previously solved AMPA and kainate receptor agonist and, antagonist complexes. This results from a hyperextension of the ligand, binding core compared with previously solved structures. As a result, in, dimer assemblies, there is a 22 A extension of the ion channel linkers in, the transition from antagonist- to glutamate-bound forms. This large, conformational change is substantially different from that described for, AMPA receptors, was not possible to predict from previous work, and, suggests that glutamate receptors are capable of much larger movements, than previously thought.

About this Structure

2F34 is a Single protein structure of sequence from Rattus norvegicus with CL, 1PE and UBA as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structures of the kainate receptor GluR5 ligand binding core dimer with novel GluR5-selective antagonists., Mayer ML, Ghosal A, Dolman NP, Jane DE, J Neurosci. 2006 Mar 15;26(11):2852-61. PMID:16540562

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