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spinqualitylabelsall models 2fa1, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of PhnF C-terminal domain

Overview

The crystal structure of Escherichia coli PhnF C-terminal domain (C-PhnF), was solved at 1.7 A resolution by the single wavelength anomalous, dispersion (SAD) method. The PhnF protein belongs to the HutC subfamily of, the large GntR transcriptional regulator family. Members of this family, share similar N-terminal DNA-binding domains, but are divided into four, subfamilies according to their heterogenic C-terminal domains, which are, involved in effector binding and oligomerization. The C-PhnF structure, provides for the first time the scaffold of this domain for the HutC, subfamily, which covers about 31% of GntR-like regulators. The structure, represents a mixture of alpha-helices and beta-strands, with a, six-stranded antiparallel beta-sheet at the core. C-PhnF monomers form a, dimer by establishing interdomain eight-strand beta-sheets that include, core antiparallel and N-terminal two-strand parallel beta-sheets from each, monomer. C-PhnF shares strong structural similarity with the chorismate, lyase fold, which features a buried active site locked behind two, helix-turn-helix loops. The structural comparison of the C-PhnF and UbiC, proteins allows us to propose that a similar site in the PhnF structure is, adapted for effector binding.

About this Structure

2FA1 is a Single protein structure of sequence from Escherichia coli with BDF as ligand. Full crystallographic information is available from OCA.

Reference

Structural characterization of GntR/HutC family signaling domain., Gorelik M, Lunin VV, Skarina T, Savchenko A, Protein Sci. 2006 Jun;15(6):1506-11. Epub 2006 May 2. PMID:16672238

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