2fz0
From Proteopedia
|
Identification of yeast R-SNARE Nyv1p as a novel longin domain protein
Overview
Using nuclear magnetic resonance spectroscopy, we establish that the, N-terminal domain of the yeast vacuolar R-SNARE Nyv1p adopts a longin-like, fold similar to those of Sec22b and Ykt6p. Nyv1p is sorted to the limiting, membrane of the vacuole via the adaptor protein (AP)3 adaptin pathway, and, we show that its longin domain is sufficient to direct transport to this, location. In contrast, we found that the longin domains of Sec22p and, Ykt6p were not sufficient to direct their localization. A YXX phi-like, adaptin-dependent sorting signal (Y31GTI34) unique to the longin domain of, Nyv1p mediates interactions with the AP3 complex in vivo and in vitro. We, show that amino acid substitutions to Y31GTI34 (Y31Q;I34Q) resulted in, mislocalization of Nyv1p as well as reduced binding of the mutant protein, to the AP3 complex. Although the sorting of Nyv1p to the limiting membrane, of the vacuole is dependent upon the Y31GTI34 motif, and Y31 in, particular, our findings with structure-based amino acid substitutions in, the mu chain (Apm3p) of yeast AP3 suggest a mechanistically distinct role, for this subunit in the recognition of YXX phi-like sorting signals.
About this Structure
2FZ0 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Identification of the yeast R-SNARE Nyv1p as a novel longin domain-containing protein., Wen W, Chen L, Wu H, Sun X, Zhang M, Banfield DK, Mol Biol Cell. 2006 Oct;17(10):4282-99. Epub 2006 Jul 19. PMID:16855025
Page seeded by OCA on Wed Nov 21 10:51:14 2007
