2gnu

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spinqualitylabelsall models 2gnu, resolution 2.200Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The crystallization of reaction center from Rhodobacter sphaeroides occurs via a new route

Overview

Bicontinuous lipidic cubic phases can be used as a host for growing, crystals of membrane proteins. Since the cubic phase is stiff, handling is, difficult and time-consuming. Moreover, the conventional cubic phase may, interfere with the hydrophilic domains of membrane proteins due to the, limited size of the aqueous pores. Here, we introduce a new, crystallization method that makes use of a liquid analogue of the cubic, phase, the sponge phase. This phase facilitates a considerable increase in, the allowed size of aqueous domains of membrane proteins, and is easily, generalised to a conventional vapour diffusion crystallisation experiment, including the use of nanoliter drop crystallization robots. The appearance, of the sponge phase was confirmed by visual inspection, small-angle X-ray, scattering and NMR spectroscopy. Crystals of the reaction centre from, Rhodobacter sphaeroides were obtained by a conventional hanging-drop, experiment, were harvested directly without the addition of lipase or, cryoprotectant, and the structure was refined to 2.2 Angstroms resolution., In contrast to our earlier lipidic cubic phase reaction centre structure, the mobile ubiquinone could be built and refined. The practical advantages, of the sponge phase make it a potent tool for crystallization of membrane, proteins.

About this Structure

2GNU is a Protein complex structure of sequences from Rhodobacter sphaeroides with FE2, CL, BCL, BPH, U10, CDL and LDA as ligands. Full crystallographic information is available from OCA.

Reference

Lipidic sponge phase crystallization of membrane proteins., Wadsten P, Wohri AB, Snijder A, Katona G, Gardiner AT, Cogdell RJ, Neutze R, Engstrom S, J Mol Biol. 2006 Nov 17;364(1):44-53. Epub 2006 Jul 7. PMID:17005199

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