2gsq
From Proteopedia
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GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE
Overview
The crystal structure of the sigma class glutathione transferase from, squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals, a third binding site for the glutathione conjugate besides the two in the, active sites of the dimer. The additional binding site is near the, crystallographic two-fold axis between the two alpha 4-turn-alpha 5, motifs. The principal binding interactions with the conjugate include, specific electrostatic interactions between the peptide and the two, subunits and a hydrophobic cavity found across the two-fold axis that, accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related, but mutually exclusive binding modes for the third conjugate are observed., The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in, the active site. This site is a potential transport binding site for, hydrophobic molecules or their glutathione conjugates.
About this Structure
2GSQ is a Single protein structure of sequence from Ommastrephes sloani with SO4 and GBI as ligands. Active as Glutathione transferase, with EC number 2.5.1.18 Full crystallographic information is available from OCA.
Reference
Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography., Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL, Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:8710848
Page seeded by OCA on Wed Nov 21 11:22:04 2007
