2gwk

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Image:2gwk.gif

2gwk, resolution 2.000Å

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SpvB ADP-ribosylated actin: orthorhombic crystal form

Overview

Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB, protein for intracellular growth and systemic virulence. SpvB covalently, modifies actin, causing cytoskeletal disruption and apoptosis. We report, here the crystal structure of the catalytic domain of SpvB, and we show by, mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting, its ATPase activity. We also describe two crystal structures of, SpvB-modified, polymerization-deficient actin. These structures reveal, that ADP-ribosylation does not lead to dramatic conformational changes in, actin, suggesting a model in which this large family of toxins inhibits, actin polymerization primarily through steric disruption of intrafilament, contacts.

About this Structure

2GWK is a Single protein structure of sequence from Oryctolagus cuniculus with CA and ATP as ligands. Full crystallographic information is available from OCA.

Reference

A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:16905096

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