2h8a
From Proteopedia
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Structure of Microsomal Glutathione Transferase 1 in Complex with Glutathione
Overview
Synthesis of mediators of fever, pain and inflammation as well as, protection against reactive molecules and oxidative stress is a hallmark, of the MAPEG superfamily (membrane associated proteins in eicosanoid and, glutathione metabolism). The structure of a MAPEG member, rat microsomal, glutathione transferase 1, at 3.2 A resolution, solved here in complex, with glutathione by electron crystallography, defines the active site, location and a cytosolic domain involved in enzyme activation. The, glutathione binding site is found to be different from that of the, canonical soluble glutathione transferases. The architecture of the, homotrimer supports a catalytic mechanism involving subunit interactions, and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.
About this Structure
2H8A is a Single protein structure of sequence from Rattus norvegicus with GTT as ligand. Active as Glutathione transferase, with EC number 2.5.1.18 Full crystallographic information is available from OCA.
Reference
Structural basis for detoxification and oxidative stress protection in membranes., Holm PJ, Bhakat P, Jegerschold C, Gyobu N, Mitsuoka K, Fujiyoshi Y, Morgenstern R, Hebert H, J Mol Biol. 2006 Jul 28;360(5):934-45. Epub 2006 Jun 5. PMID:16806268
Page seeded by OCA on Wed Nov 21 11:35:59 2007
