This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2hdc
From Proteopedia
|
STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX
Overview
Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR, techniques, we have obtained the solution structure and backbone dynamics, of Genesis complexed with a 17 base-pair DNA. Our results indicate that, both the local folding and dynamic properties of Genesis are perturbed, when it binds to the DNA site. Our data show that a conserved flexible, amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex, and a short helix is induced by Genesis-DNA interactions. Our data, indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not, required in forming the stable Genesis-DNA complex.
About this Structure
2HDC is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex., Jin C, Marsden I, Chen X, Liao X, J Mol Biol. 1999 Jun 18;289(4):683-90. PMID:10369754
Page seeded by OCA on Wed Nov 21 11:41:12 2007
Categories: Rattus norvegicus | Single protein | Chen, X. | Jin, C. | Liao, X. | Marsden, I. | Dyanamics | Genesis | Nmr | Structure | Winged helix protein
