2het
From Proteopedia
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Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3
Overview
Recoverin is a Ca(2+)-regulated signal transduction modulator expressed in, the vertebrate retina that has been implicated in visual adaptation. An, intriguing feature of recoverin is a cluster of charged residues at its C, terminus, the functional significance of which is largely unclear. To, elucidate the impact of this segment on recoverin structure and function, we have investigated a mutant lacking the C-terminal 12 amino acids., Whereas in myristoylated recoverin the truncation causes an overall, decrease in Ca(2+) sensitivity, results for the non-myristoylated mutant, indicate that the truncation primarily affects the high affinity EF-hand, 3. The three-dimensional structure of the mutant has been determined by, x-ray crystallography. In addition to significant changes in average, coordinates compared with wild-type recoverin, the structure provides, strong indication of increased conformational flexibility, particularly in, the C-terminal domain. Based on these observations, we propose a novel, role of the C-terminal segment of recoverin as an internal modulator of, Ca(2+) sensitivity.
About this Structure
2HET is a Single protein structure of sequence from Bos taurus with CA as ligand. Full crystallographic information is available from OCA.
Reference
Tuning of a neuronal calcium sensor., Weiergraber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2006 Dec 8;281(49):37594-602. Epub 2006 Oct 2. PMID:17015448
Page seeded by OCA on Wed Nov 21 11:42:20 2007
