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2ca6
From Proteopedia
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MIRAS STRUCTURE DETERMINATION FROM HEMIHEDRALLY TWINNED CRYSTALS
Overview
The structure of Rna1p was originally solved to 2.7 A resolution by MIRAS, from crystals with partial hemihedral twinning in space group I4(1), [Hillig et al. (1999), Mol. Cell, 3, 781-791] by finding a low-twinned, native crystal (twin fraction alpha=0.06) and after twin correction of all, data sets. Rna1p crystals have now been used to examine how far twinning, and twin correction affect MIR phasing with a higher resolution but highly, twinned native data set. Even high hemihedral twinning [alphanative=0.39, alphaderivative=0.24] would not have hindered heavy-atom site, identification of strong derivatives using difference Patterson maps., However, a weaker derivative could have been missed and refinement would, have stalled at high R values had twinning not been identified and, ... [(full description)]
About this Structure
2CA6 is a [Single protein] structure of sequence from [Schizosaccharomyces pombe] with SO4 as [ligand]. Full crystallographic information is available from [OCA].
Reference
Detecting and overcoming hemihedral twinning during the MIR structure determination of Rna1p., Hillig RC, Renault L, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):750-65. Epub 2006, Jun 20. PMID:16790931
Page seeded by OCA on Mon Oct 29 22:07:42 2007
Categories: Schizosaccharomyces pombe | Single protein | Hillig, R.C. | Renault, L. | SO4 | Gap | Gtpase activation | Gtpase-activating protein | Hemihedral twinning | Leucine-rich repeat protein | Lrr | Merohedral twinning | Merohedry | Nuclear transport | Rangap | Rna1p | Signaling activator | Signaling protein | Signaling regulator
