2i5p
From Proteopedia
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Crystal structure of glyceraldehyde-3-phosphate dehydrogenase isoform 1 from K. marxianus
Overview
The presence of an isoform of glyceraldehyde-3-phosphate dehydrogenase, (kmGAPDH1p) associated with the cell wall of a flocculent strain of, Kluyveromyces marxianus was the first report of a non-cytosolic, localization of a glycolytic enzyme, but the mechanism by which the, protein is transported to the cell surface is not known. To identify, structural features that could account for the multiple localizations of, the protein, the three-dimensional structure of kmGAPDH1p was determined, by x-ray crystallography and small angle x-ray scattering. The x-ray, crystallographic structure of kmGAPDH1p revealed a dimer, although all, GAPDH homologs studied thus far have a tetrameric structure with 222, symmetry. Interestingly, the structure of kmGAPDH1p in solution revealed a, tetramer with a 70 degrees tilt angle between the dimers. Moreover, the, separation between the centers of the dimers composing the kmGAPDH1p, tetramer diminished from 34 to 30 A upon NAD(+) binding, this latter value, being similar to the observed in the crystallographic models of GAPDH, homologs. The less compact structure of apo-kmGAPDH1p could already be the, first image of the transition intermediate between the tetramer observed, in solution and the dimeric form found in the crystal structure, which we, postulate to exist in vivo because of the protein's multiple subcellular, localizations in this yeast species.
About this Structure
2I5P is a Single protein structure of sequence from Kluyveromyces marxianus with GLC and BME as ligands. Active as Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating), with EC number 1.2.1.12 Full crystallographic information is available from OCA.
Reference
The crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures., Ferreira-da-Silva F, Pereira PJ, Gales L, Roessle M, Svergun DI, Moradas-Ferreira P, Damas AM, J Biol Chem. 2006 Nov 3;281(44):33433-40. Epub 2006 Sep 7. PMID:16963457
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