2iwm

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Image:2iwm.jpg

2iwm, resolution 2.50Å

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PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS

Overview

The crystallization of three catalytically inactive mutants of penicillin, V acylase (PVA) from Bacillus sphaericus in precursor and processed forms, is reported. The mutant proteins crystallize in different primitive, monoclinic space groups that are distinct from the crystal forms for the, native enzyme. Directed mutants and clone constructs were designed to, study the post-translational autoproteolytic processing of PVA. The, catalytically inactive mutants will provide three-dimensional structures, of precursor PVA forms, plus open a route to the study of enzyme-substrate, complexes for this industrially important enzyme.

About this Structure

2IWM is a Single protein structure of sequence from Lysinibacillus sphaericus. Active as Penicillin amidase, with EC number 3.5.1.11 Full crystallographic information is available from OCA.

Reference

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:16508111

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