2o0i
From Proteopedia
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crystal structure of the R185A mutant of the N-terminal domain of the Group B Streptococcus Alpha C protein
Overview
Group B Streptococcus (GBS) frequently colonizes the human, gastrointestinal and gynecological tracts and less frequently causes deep, tissue infections. The transition between colonization and infection, depends upon the ability of the organism to cross epithelial barriers. The, alpha C protein (ACP) on the surface of GBS contributes to this process. A, virulence factor in mouse models of infection, and prototype for a family, of Gram-positive bacterial surface proteins, ACP facilitates GBS entry, into human cervical epithelial cells and movement across cell layers. ACP, binds to host cell surface glycosaminoglycan (GAG). From crystallography, we have identified a cluster of basic residues (BR2) that is a putative, GAG binding area in Domain 2, near the junction of the N-terminal domain, of ACP and the first of a series of tandem amino acid repeats. D2-R, a, protein construct including this region, binds to cells similarly to, full-length ACP. We now demonstrate that the predicted charged BR2, residues confer GAG binding; site-directed mutagenesis of these residues, (Arg(172), Arg(185), or Lys(196)) eliminates cell-binding activity of, construct D2-R. In addition, we have constructed a GBS strain expressing a, variant ACP with a charge-neutralizing substitution at residue 185. This, strain enters host cells less effectively than does the wild-type strain, and similarly to an ACP null mutant strain. The point mutant strain, transcytoses similarly to the wild-type strain. These data indicate that, GAG-binding activity underlies ACP-mediated cellular entry of GBS. GBS, entry into host cells and transcytosis of host cells may occur by distinct, mechanisms.
About this Structure
2O0I is a Single protein structure of sequence from Streptococcus agalactiae serogroup iii. Full crystallographic information is available from OCA.
Reference
Identification of a glycosaminoglycan binding region of the alpha C protein that mediates entry of group B streptococci into host cells., Baron MJ, Filman DJ, Prophete GA, Hogle JM, Madoff LC, J Biol Chem. 2007 Apr 6;282(14):10526-36. Epub 2007 Jan 26. PMID:17259175
Page seeded by OCA on Wed Nov 21 13:00:47 2007
