2o4c

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spinqualitylabelsall models 2o4c, resolution 2.300Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of D-Erythronate-4-phosphate Dehydrogenase Complexed with NAD

Overview

Pyridoxal-5'-phosphate (the active form of vitamin B6) is an essential, cofactor in many enzymatic reactions. While animals lack any of the, pathways for de novo synthesis and salvage of vitamin B6, it is, synthesized by two distinct biosynthetic routes in bacteria, fungi, parasites, and plants. One of them is the PdxA/PdxJ pathway found in the, gamma subdivision of proteobacteria. It depends on the pdxB gene, which, encodes erythronate-4-phosphate dehydrogenase (PdxB), a member of the, d-isomer specific 2-hydroxyacid dehydrogenase superfamily. Although, three-dimensional structures of other functionally related dehydrogenases, are available, no structure of PdxB has been reported. To provide the, missing structural information and to gain insights into the catalytic, mechanism, we have determined the first crystal structure of, erythronate-4-phosphate dehydrogenase from Pseudomonas aeruginosa in the, ligand-bound state. It is a homodimeric enzyme consisting of 380-residue, subunits. Each subunit consists of three structural domains: the lid, domain, the nucleotide-binding domain, and the C-terminal dimerization, domain. The latter domain has a unique fold and is largely responsible for, dimerization. Interestingly, two subunits of the dimeric enzyme are bound, with different combinations of ligands in the crystal and they display, significantly different conformations. Subunit A is bound with NAD and a, phosphate ion, while subunit B, with a more open active site cleft, is, bound with NAD and l(+)-tartrate. Our structural data allow a detailed, understanding of cofactor and substrate recognition, thus providing, substantial insights into PdxB catalysis.

About this Structure

2O4C is a Single protein structure of sequence from Pseudomonas aeruginosa with PO4, NAD, TLA and GOL as ligands. Active as 4-phosphoerythronate dehydrogenase, with EC number 1.1.1.290 Full crystallographic information is available from OCA.

Reference

Crystal Structure of d-Erythronate-4-phosphate Dehydrogenase Complexed with NAD., Ha JY, Lee JH, Kim KH, Kim do J, Lee HH, Kim HK, Yoon HJ, Suh SW, J Mol Biol. 2007 Mar 2;366(4):1294-304. Epub 2006 Dec 19. PMID:17217963

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