2owo

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Image:2owo.gif

2owo, resolution 2.300Å

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Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate

Overview

NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria and, essential for growth. Their distinctive substrate specificity and domain, organization vis-a-vis human ATP-dependent ligases make them outstanding, targets for anti-infective drug discovery. We report here the 2.3 A, crystal structure of Escherichia coli LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the, basis for nick recognition. LigA envelops the DNA within a protein clamp., Large protein domain movements and remodeling of the active site, orchestrate progression through the three chemical steps of the ligation, reaction. The structure inspires a strategy for inhibitor design.

About this Structure

2OWO is a Single protein structure of sequence from Escherichia coli with ZN, SO4 and AMP as ligands. Active as DNA ligase (NAD(+)), with EC number 6.5.1.2 Full crystallographic information is available from OCA.

Reference

Last Stop on the Road to Repair: Structure of E. coli DNA Ligase Bound to Nicked DNA-Adenylate., Nandakumar J, Nair PA, Shuman S, Mol Cell. 2007 Apr 27;26(2):257-71. PMID:17466627

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