2jcd

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spinqualitylabelsall models 2jcd, resolution 2.11Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF THE N-OXYGENASE AURF FROM STREPTOMYCES THIOLUTEUS

Overview

Nitro groups are found in a number of bioactive compounds. Most of them, arise by a stepwise mono-oxygenation of amino groups. One of the involved, enzymes is AurF participating in the biosynthesis of aureothin. Its, structure was established at 2.1 A resolution showing a homodimer with a, binuclear manganese cluster. The enzyme preparation, which yielded the, analyzed crystals, showed activity using in vitro and in vivo assays., Chain fold and cluster are homologous with ribonucleotide reductase, subunit R2 and related enzymes. The two manganese ions and an iron content, of about 15% were established by anomalous X-ray diffraction. A comparison, of the cluster with more common di-iron clusters suggested an additional, histidine in the coordination sphere to cause the preference for ... [(full description)]

About this Structure

2JCD is a [Single protein] structure of sequence from [Streptomyces thioluteus] with MN and EDO as [ligands]. Full crystallographic information is available from [OCA].

Reference

Structure and Action of the N-oxygenase AurF from Streptomyces thioluteus., Zocher G, Winkler R, Hertweck C, Schulz GE, J Mol Biol. 2007 Jun 9;. PMID:17765264

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